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In Vivo And In Vitro Toxicity of Binary Combination of Column Purified Fraction of Asparagus Racemosus and Glycyrrhiza Glabra Against the Sporocyst, Redia and Cercaria Larva of Fasciola Gigantica

A. K. Vishwakarma1, Ravikash Maurya and Pradeep Kumar3
Departmrnt of Zoology, Doodhnath Singh Smarak Mahavidyalaya Mariahu,
Jaunpur U.P. 222161; Email: akhi.vk1990@gmail.com
Department of Zoology, Kutir P.G. College Chakkey, Jaunpur U.P. 222146
Email: mr.ravikash@gmail.com; ORCID: 0009-0002-4300-0222
Department of Zoology, D.D.U. Govt. Degree College Sahjanwa,
Gorakhpur U.P. 273209

*Address for correspondence
Dr. Pradeep Kumar
Department of Zoology
D.D.U. Govt. Degree College Sahjanwa,
Gorakhpur U.P. 273209
Email: akhi.vk1990@gmail.com,

Abstract
In binary combination (1:1) of the activity of A. racemosus and G. glabra against Fasciola gigantica larva in Lymnaea acuminate observe in vivo and in vitro experiments, which is based on time and concentration-dependent toxicity. In vivo treatment represent the highest toxicity against cercaria larva, with 2Hr LC50 and 8Hr LC50 of A. racemosus and G. glabra at 64.85 mg/L and 39.78 mg/L, respectively while in in vitro treatment the toxicity against sporocyst larva, with 2Hr and 8Hr LC50 values of 64.59 mg/ml and 46.27 mg/ml, respectively. Negative regression between exposure period and LC50 is significant (p<0.05). Steep slope values, t-ratios >1.96, and g values <0.5 shows reliability of the data. This study suggests the potential of A. racemosus and G. glabra as larvicidal plant products against F. gigantica, offering a novel
approach to control fascioliasis without harming intermediate host snails.
Key words: Fasciola gigantica, Fascioliasis, Asparagus racemosus, Glycyrrhiza glabra.