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Development and method validation of RP-HPLC Method- A Review

Dr. J. SAI CHANDRA

Assistant Professor, Dept. of Chemistry, JNTUH University College of Engineering Sultanpur, Sangareddy, Telangana, INDIA- 502273.

ANITHA NALLAMOTHU

 Assistant Professor (c), UCEN-JNTUK Narasaraopet, Andhra pradesh, INDIA- 522 601.

Dr V L N Murthy KAMAVARAPU

Lecturer, Dept. of Chemistry, SVR DEGREE COLLEGE, Macherla, Andhra Pradesh- 522 426

Abstract:
The development of RP HPLC methods is not without difficulties, despite its many advantages. These difficulties include problems with column stability, sample preparation, and optimization. This article describes new techniques that can be used with the RP-HPLC process to design and produce novel pharmaceuticals. Additionally covered are instances of more current developments in hyphenated methods like RP-HPLC. We intend to stimulate ongoing innovation and advancement in the developing field of pharmacy by examining the most recent developments, applications, and difficulties in the creation of RP-HPLC methods.

Keywords: RP-HPLC, LC-MS and API (Active Pharmaceutical Ingredient)