Estimation of Riboflavin in Pharmaceutical Drugs using UV/Vis Spectrophotometry and Confirmation of the Results by RP-HPLC

Authors:

Khilari Kishor Annasaheb

Department of Chemistry, Loknete Ramdas Patil Dhumal Art's Science and Commerce College Rahuri, Ahilyanagar

Corresponding author: kishorkhilari2@gmail.com

Abstract - A rapid, simple, sensitive, isocratic, reproducible reversed-phase HPLC technique has been developed for the quantitative assay evaluation of Vitamin D₃ in various pharmaceutical drugs. Vitamins and Dietary supplements are essential to maintain the health of the human body. As it is fat-soluble, it is incorporated into cell membranes, which protects them from oxidative damage. However, other functions have also been recognized to be of importance. The simple, precise, rapid, and accurate UV/Vis-Spectrophotometry and RP-HPLC method has been developed for the estimation of Riboflavin (Vit B₂) in tablet dosage from Riboflavin (Vit B₂) assayed in the pharmaceutical pure form. The vitamin B2 is the water-soluble vitamin in the present study the wavelength selected for the analysis was λ_max at 448 nm in aqueous media. The equation of the line for Absorbance versus Concentration was y = 0.0275x + 0.0237 (intercept at origin), and the regression R-squared coefficient was 0.9992 (R² = 0.9992). The assay for riboflavin in an aqueous mixture shows a high level of accuracy and sensitivity. HPLC analyses are mainly performed on C₁₈ stationary Reverse-Phase (RP) with hydrophilic eluents based on water-methanol, followed by ultraviolet (UV) detection. The equation of the line for Peak Area versus Concentration was y = 130338x + 135019 (intercept at origin), and the regression correlation coefficient was 0.9994 (R² = 0.9994).

Keywords: Cholecalciferol, RP- HPLC, Double Beam Spectrophotometry, Method Validation.